Journal article

Ischemic Injury Does Not Stimulate Striatal Neuron Replacement Even during Periods of Active Striatal Neurogenesis

Charlotte M Ermine, Jordan L Wright, Davor Stanic, Clare L Parish, Lachlan H Thompson

iScience | CELL PRESS | Published : 2020

Abstract

Ischemic damage to the adult rodent forebrain has been widely used as a model system to study injury-induced neurogenesis, resulting in contradictory reports regarding the capacity of the postnatal brain to replace striatal projection neurons. Here we used a software-assisted, confocal approach to survey thousands of cells generated after striatal ischemic injury in rats and showed that injury fails not only to stimulate production of new striatal projection neurons in the adult brain but also to do so in the neonatal brain at early postnatal ages not previously explored. Conceptually this is significant, because it shows that even during periods of active striatal neurogenesis, injury is no..

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Grants

Awarded by NHMRC


Funding Acknowledgements

The authors thank Mong Tien for expert technical assistance in the tissue preparation and immunohistochemical procedures. C.L.P. is an NHMRC Senior Research Fellow. This work was supported NHMRC project grant 1130734. The Florey Institute of Neuroscience and Mental Health acknowledges the strong support from the Victorian Government and in particular the funding from the Operational Infrastructure Support Grant.