Journal article
Snail induces epithelial cell extrusion by regulating RhoA contractile signalling and cell-matrix adhesion
Kenneth Wee, Soroor Hediyeh-Zadeh, Kinga Duszyc, Suzie Verma, Bageshri N Nanavati, Satyajeet Khare, Amrita Varma, Roger J Daly, Alpha S Yap, Melissa J Davis, Srikanth Budnar
Journal of Cell Science | COMPANY BIOLOGISTS LTD | Published : 2020
DOI: 10.1242/jcs.235622
Abstract
Cell extrusion is a morphogenetic process that is implicated in epithelial homeostasis and elicited by stimuli ranging from apoptosis to oncogenic transformation. To explore whether the morphogenetic transcription factor Snail (SNAI1) induces extrusion, we inducibly expressed a stabilized Snail6SA transgene in confluent MCF-7 monolayers. When expressed in small clusters (less than three cells) within otherwise wild-type confluent monolayers, Snail6SA expression induced apical cell extrusion. In contrast, larger clusters or homogenous cultures of Snail6SA cells did not show enhanced apical extrusion, but eventually displayed sporadic basal delamination. Transcriptomic profiling revealed that ..
View full abstractGrants
Awarded by Queensland Cancer Council
Awarded by National Health and Medical Research Council of Australia
Awarded by National Breast Cancer Foundation
Awarded by Australian Research Council Center of Excellence in Convergent Bio-Nano Science and Technology
Awarded by NHMRC Fellowship
Funding Acknowledgements
This work was supported by an Australian Postgraduate Award, to K.W., and grants and fellowships from the Queensland Cancer Council (1086587, 112823), and the National Health and Medical Research Council of Australia (1044041, 1136592, 1067405) to A.S.Y. M.J.D. is supported by National Breast Cancer Foundation (ECF-14-043 and CG-10-04; funding of the EMPathy Breast Cancer Network) and the Australian Research Council Center of Excellence in Convergent Bio-Nano Science and Technology (project number CE140100036). R.J.D. is supported by NHMRC Fellowship APP1058540.