Journal article

Snail induces epithelial cell extrusion by regulating RhoA contractile signalling and cell-matrix adhesion

Kenneth Wee, Soroor Hediyeh-Zadeh, Kinga Duszyc, Suzie Verma, Bageshri N Nanavati, Satyajeet Khare, Amrita Varma, Roger J Daly, Alpha S Yap, Melissa J Davis, Srikanth Budnar

Journal of Cell Science | COMPANY BIOLOGISTS LTD | Published : 2020

Abstract

Cell extrusion is a morphogenetic process that is implicated in epithelial homeostasis and elicited by stimuli ranging from apoptosis to oncogenic transformation. To explore whether the morphogenetic transcription factor Snail (SNAI1) induces extrusion, we inducibly expressed a stabilized Snail6SA transgene in confluent MCF-7 monolayers. When expressed in small clusters (less than three cells) within otherwise wild-type confluent monolayers, Snail6SA expression induced apical cell extrusion. In contrast, larger clusters or homogenous cultures of Snail6SA cells did not show enhanced apical extrusion, but eventually displayed sporadic basal delamination. Transcriptomic profiling revealed that ..

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University of Melbourne Researchers

Grants

Awarded by Queensland Cancer Council


Awarded by National Health and Medical Research Council of Australia


Awarded by National Breast Cancer Foundation


Awarded by Australian Research Council Center of Excellence in Convergent Bio-Nano Science and Technology


Awarded by NHMRC Fellowship


Funding Acknowledgements

This work was supported by an Australian Postgraduate Award, to K.W., and grants and fellowships from the Queensland Cancer Council (1086587, 112823), and the National Health and Medical Research Council of Australia (1044041, 1136592, 1067405) to A.S.Y. M.J.D. is supported by National Breast Cancer Foundation (ECF-14-043 and CG-10-04; funding of the EMPathy Breast Cancer Network) and the Australian Research Council Center of Excellence in Convergent Bio-Nano Science and Technology (project number CE140100036). R.J.D. is supported by NHMRC Fellowship APP1058540.