Biomaterials functionalized with nanoclusters of integrin- and syndecan-binding ligands improve cell adhesion and mechanosensing under shear flow conditions
Fatemeh Karimi, Varsha Jagannath Thombare, Craig A Hutton, Andrea J O'Connor, Greg G Qiao, Daniel E Heath
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | WILEY | Published : 2020
We have engineered biomaterials that display nanoclusters of ligands that bind both integrin and syndecan-4 cell receptors. These surfaces regulate cell behaviors under static conditions including adhesion, spreading, actin stress fiber formation, and migration. The syndecan-4 receptors are also critical mediators of cellular mechanotransduction. In this contribution we assess whether this novel class of materials can regulate the response of cells to applied mechanical stimulation, using the shear stress imparted by laminar fluid flow as a model stimulus. Specifically, we assess endothelial cell detachment due to flow, cell alignment due to flow, and cell adhesion from the flowing fluid. A ..View full abstract
The authors would like to thank the Research Training Program (RTP), the Endeavor IPRS, and the Australian Postgraduate Awards (APA) for providing financial support to this project. We would also like to thank the Particulate Fluids Processing Centre (PFPC) and the Materials Characterisation and Fabrication Platform (MCFP).