Increased MUC1 plus a larger quantity and complex size for MUC5AC in the peripheral airway lumen of long-term tobacco smokers.
Médea Padra, Anders Andersson, Bettina Levänen, Pushpa Premaratne, Helga Asgeirsdottir, Sara Tengvall, Karin Christenson, Marit Stockfelt, Steven Bozinovski, Shigemi Yoshihara, Ann-Sofie Lantz, Lena Palmberg, Barbro Dahlén, Kjell Larsson, Ingemar Qvarfordt, Sara K Lindén, Anders Lindén
Clin Sci (Lond) | Published : 2020
There is little information on mucins versus potential regulatory factors in the peripheral airway lumen of long-term smokers with (LTS+) and without (LTS-) chronic obstructive pulmonary disease (COPD). We explored these matters in bronchoalveolar lavage (BAL) samples from two study materials, both including LTS+ and LTS- with a very similar historic exposure to tobacco smoke, and healthy non-smokers (HNSs; n=4-20/group). Utilizing slot blot and immunodetection of processed (filtered and centrifuged), as well as unprocessed BAL samples from one of the materials, we compared the quantity and fraction of large complexes of mucins. All LTS displayed an enhanced (median) level of MUC5AC compared..View full abstract