Leishmania Encodes a Bacterium-like 2,4-Dienoyl-Coenzyme A Reductase That Is Required for Fatty Acid beta-Oxidation and Intracellular Parasite Survival
Geo Semini, Daniel Paape, Martin Blume, M Fleur Sernee, Diego Peres-Alonso, Sebastien Calvignac-Spencer, Joerg Doellinger, Stefan Jehle, Eleanor Saunders, Malcolm J McConville, Toni Aebischer
mBio | AMER SOC MICROBIOLOGY | Published : 2020
Leishmania spp. are protozoan parasites that cause a spectrum of important diseases in humans. These parasites develop as extracellular promastigotes in the digestive tract of their insect vectors and as obligate intracellular amastigotes that infect macrophages and other phagocytic cells in their vertebrate hosts. Promastigote-to-amastigote differentiation is associated with marked changes in metabolism, including the upregulation of enzymes involved in fatty acid β-oxidation, which may reflect adaptation to the intracellular niche. Here, we have investigated the function of one of these enzymes, a putative 2,4-dienoyl-coenzyme A (CoA) reductase (DECR), which is specifically required for th..View full abstract
Awarded by Federal Ministry of Education and Research (BMBF)
Awarded by European Commission Marie Curie Excellence grant
Awarded by Deutsche Forschungsgemeinschaft
M.J.M. is a NHMRC Principal Research Fellow. The SP8 microscope was maintained by Biological Optical Microscopy Platform (BOMP), Faculty of Medicine, Dentistry & Health Sciences, the University of Melbourne, Victoria, Australia. Ellie Hyun-Jung Cho, Applications Specialist of BOMP, has provided technical assistance with quantitation of microscopy data. M.B. was funded by the Federal Ministry of Education and Research (BMBF) under project number 01KI1715 as part of the "Research Network Zoonotic Infectious Diseases" and by a DFG research fellowship. This work was supported by European Commission Marie Curie Excellence grant Ext-25435 and by Deutsche Forschungsgemeinschaft grant AE16/5-1,2, both to T.A.