Journal article
Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells
M Nafria, P Keane, ES Ng, EG Stanley, AG Elefanty, C Bonifer
Cell Reports | CELL PRESS | Published : 2020
Abstract
This study uses human-embryonic-stem-cell-derived definitive human blood progenitors to gain insight into the earliest events following leukemic oncogene expression. Nafria et al. show that induction of RUNX1-ETO expression rapidly reprograms chromatin, disrupts global RUNX1 binding, and arrests growth and/or differentiation specifically in early myeloid progenitor cells.
Grants
Awarded by Children's Cancer Foundation
Funding Acknowledgements
This work was funded by a U21 Melbourne studentship and a Wellcome Trust Institutional Strategic Support Fund (ISSF) grant to the University of Birmingham held by M.N., as well as a grant from the Birmingham Cancer Research UK (CRUK) Centre to C.B. We thank the Birmingham Next Generation Sequencing Facility (Genomics Birmingham) for expert service. M.N. was awarded The Henry and Rachael Ackman Travelling Scholarship by the University of Melbourne Faculty of Medicine, Dentistry and Health Sciences. Work in the laboratories of A.G.E. and E. G.S. was funded by the National Health and Medical Research Council of Australia (GNT1068866, GNT1129861, and GNT1138717), the Australian Research Council Special Research Initiative in Stem Cells (Stem Cells Australia), the Children's Cancer Foundation, and the Stafford Fox Medical Research Foundation. E.G. S. (GNT1079004) and A.G.E. (GNT1117596) are research fellows of National Health and Medical Research Council (Australia). Additional infrastructure funding to the Murdoch Children's Research Institute was provided by the Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme and the Victorian government's Operational Infrastructure Support Program.