Journal article

Phase-plate cryo-EM structure of a class B GPCR-G-protein complex

Yi-Lynn Liang, Maryam Khoshouei, Mazdak Radjainia, Yan Zhang, Alisa Glukhova, Jeffrey Tarrasch, David M Thal, Sebastian GB Furness, George Christopoulos, Thomas Coudrat, Radostin Danev, Wolfgang Baumeister, Laurence J Miller, Arthur Christopoulos, Brian K Kobilka, Denise Wootten, Georgios Skiniotis, Patrick M Sexton

Nature | NATURE PUBLISHING GROUP | Published : 2017

Grants

Awarded by National Health and Medical Research Council of Australia (NHMRC)


Awarded by NIH


Awarded by Melbourne Bioinformatics at the University of Melbourne


Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Awarded by NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE


Funding Acknowledgements

This work was funded by the National Health and Medical Research Council of Australia (NHMRC) (grant numbers 1055134, 1061044 and 1120919) and NIH grants DK090165, NS092695. P.M.S. and A.C. are NHMRC Principal and Senior Principal Research Fellows, respectively. D.W. is a NHMRC Career Development Fellow. Computational studies were partially supported by Melbourne Bioinformatics at the University of Melbourne, grant number VR0024. Negative-stain imaging and cryo-EM screening was performed at the Monash Ramaciotti Centre for Cryo-Electron Microscopy. The GPCRdb (http://gpcrdb.org) was used for generation of initial alignments of human class B GPCR sequences. The authors thank the late M. Azria for 100 mg of sCT, used in initial work.