Unveiling the Membrane-Binding Properties of N-Terminal and C-Terminal Regions of G Protein-Coupled Receptor Kinase 5 by Combined Optical Spectroscopies
Bei Ding, Alisa Glukhova, Katarzyna Sobczyk-Kojiro, Henry I Mosberg, John JG Tesmer, Zhan Chen
Langmuir: the ACS journal of surfaces and colloids | AMER CHEMICAL SOC | Published : 2014
G protein-coupled receptor kinase 5 (GRK5) is thought to associate with membranes in part via N- and C-terminal segments that are typically disordered in available high-resolution crystal structures. Herein we investigate the interactions of these regions with model cell membrane using combined sum frequency generation (SFG) vibrational spectroscopy and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. It was found that both regions associate with POPC lipid bilayers but adopt different structures when doing so: GRK5 residues 2-31 (GRK5(2-31)) was in random coil whereas GRK5(546-565) was partially helical. When the subphase for the GRK5(2-31) peptide was change..View full abstract
Awarded by National Institute of Health
Awarded by NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
The work is supported by the National Institute of Health grants GM081655 (to Z.C.) and HL071818 (to J.J.G.T.). B.D. acknowledges the financial support of Barbour Fellowship from the University of Michigan. We genuinely thank Dr. Pei Yang for technical support.