A Targeted "Next-Generation" Sequencing-Informatic Approach to Define Genetic Diversity in Theileria orientalis Populations within Individual Cattle: Proof-of-Principle

Anson Koehler; Abdul Jabbar; Ross S Hall; Robin B Gasser

Journal article

A Targeted "Next-Generation" Sequencing-Informatic Approach to Define Genetic Diversity in Theileria orientalis Populations within Individual Cattle: Proof-of-Principle

Anson Koehler, Abdul Jabbar, Ross S Hall, Robin B Gasser

Pathogens | MDPI | Published : 2020

DOI: 10.3390/pathogens9060448

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Abstract

Oriental theileriosis is an economically important tickborne disease of bovines, caused by some members of the Theileria orientalis complex. Currently, 11 distinct operational taxonomic units (OTUs), or genotypes, are recognized based on their major piroplasm surface protein (MPSP) gene sequences. Two of these genotypes (i.e., chitose and ikeda) are recognized as pathogenic in cattle, causing significant disease in countries of the Asia-Pacific region. However, the true extent of genetic variation and associated virulence/pathogenicity within this complex is unknown. Here, we undertook a proof-of-principle study of a small panel of genomic DNAs (n = 13) from blood samples originating from in..

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University of Melbourne Researchers

Abdul Jabbar Author Veterinary Biosciences

Anson Koehler Author Veterinary Biosciences

Robin Gasser Author Veterinary Biosciences

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Awarded by Australian Research Council

Funding Acknowledgements

This study was supported through a Collaborative Research Grant (no. 501101 to A.J.), an Early Career Research Grant (no. 601308 to A.J.) from The University of Melbourne, and grants from the Australian Research Council (LP180101085 to R.B.G. and LP160101299 to R.B.G. and A.V.K.).