CIRCADIAN AND WAKE-DEPENDENT CHANGES IN THE HUMAN PLASMA METABOLOME

Abstract

Several studies, using group level analyses, have identified plasma metabolites that display 24-hour rhythms and/or change with time awake. Inter-individual differences in these patterns have not been examined in detail, however. The aim of the current study was to assess endogenous self-sustained circadian rhythms and wake-dependent changes in the human plasma metabolome under highly controlled conditions. Thirteen healthy participants aged 20–32 years (9 males) were studied during a 40h constant routine (CR). Four-hourly plasma samples were analyzed using hydrophilic interaction liquid chromatography coupled with mass spectrometry (HILIC/MS) in order to conduct an untargeted analysis of po..