Quantum diffraction unlimited protocol for single-photon fluorophores
Josef G Worboys, Daniel W Drumm, Andrew D Greentree, EM Goldys (ed.), BC Gibson (ed.)
BIOPHOTONICS AUSTRALASIA 2019 | SPIE-INT SOC OPTICAL ENGINEERING | Published : 2019
Achieving higher resolution scales in optical microscopy allow a more rigorous investigation into the detailed components of cell systems. This higher resolution is typically achieved through super-resolution techniques utilizing methods inside the wave-like nature of light such as point spread function shaping and fluorophore switching. We wish to leverage both particle-like and wave-like natures of light to make a diffraction unlimited protocol. Our protocol uses the well known Hanbury Brown and Twiss (HBT) apparatus in combination with a customized second-order cross-correlation protocol. By performing least squares fitting of the HBT and intensity measurements we obtain diffraction unlim..View full abstract
Awarded by ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP)
Awarded by ARC Future Fellowship
Awarded by Australian Government
The authors acknowledge the support of the ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP) (Grant No. CE140100003), ADG acknowledges the support of an ARC Future Fellowship (Grant No. FT160100357). This research was undertaken with the assistance of resources and services from the National Computational Infrastructure (NCI), which is supported by the Australian Government (LE160100051).