First in-human study of in vivo imaging of ex vivo labeled CAR T cells with dual PET-MR.

Ritu Singla; Dominic M Wall; Samuel Anderson; Nicholas Zia; James C Korte; Lucy Kravets; Gerard McKiernan; Jeanne Butler; Amanda Gammilonghi; Jyoti Arora; Ben J Solomon; Rodney J Hicks; Timothy Cain; Phillip K Darcy; Carleen Cullinane; Paul J Neeson; Rajesh Ramanathan; Ravi Shukla; Vipul Bansal; Simon J Harrison

Journal article

First in-human study of in vivo imaging of ex vivo labeled CAR T cells with dual PET-MR.

Ritu Singla, Dominic M Wall, Samuel Anderson, Nicholas Zia, James C Korte, Lucy Kravets, Gerard McKiernan, Jeanne Butler, Amanda Gammilonghi, Jyoti Arora, Ben J Solomon, Rodney J Hicks, Timothy Cain, Phillip K Darcy, Carleen Cullinane, Paul J Neeson, Rajesh Ramanathan, Ravi Shukla, Vipul Bansal, Simon J Harrison

JOURNAL OF CLINICAL ONCOLOGY | LIPPINCOTT WILLIAMS & WILKINS | Published : 2020

DOI: 10.1200/jco.2020.38.15_suppl.3557

Abstract

3557 Background: This is a first in human in-vivo biodistribution of ex-vivo labelled CAR T cells assessed in a cohort of patients. Cells were labelled with novel Cu-64 labelled superparamagnetic iron oxide nanoparticles (SPION) and infused IV into patients with solid tumors & tracked using clinical dual PET-MR. The study validates the clinical translation of CAR T cell in-vivo tracking in real time. Methods: Cu-64 radioisotope was bound to silica coated SPION using electrolysis plating with tin & palladium seeding. Cellular uptake of Cu-64 SPION was facilitated with a transfecting agent. Functional assays including 51Chromium release, cytometric bead array demonstrated that labelling proce..

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