First in-human study of in vivo imaging of ex vivo labeled CAR T cells with dual PET-MR.
Ritu Singla, Dominic M Wall, Samuel Anderson, Nicholas Zia, James C Korte, Lucy Kravets, Gerard McKiernan, Jeanne Butler, Amanda Gammilonghi, Jyoti Arora, Ben J Solomon, Rodney J Hicks, Timothy Cain, Phillip K Darcy, Carleen Cullinane, Paul J Neeson, Rajesh Ramanathan, Ravi Shukla, Vipul Bansal, Simon J Harrison
JOURNAL OF CLINICAL ONCOLOGY | LIPPINCOTT WILLIAMS & WILKINS | Published : 2020
3557 Background: This is a first in human in-vivo biodistribution of ex-vivo labelled CAR T cells assessed in a cohort of patients. Cells were labelled with novel Cu-64 labelled superparamagnetic iron oxide nanoparticles (SPION) and infused IV into patients with solid tumors & tracked using clinical dual PET-MR. The study validates the clinical translation of CAR T cell in-vivo tracking in real time. Methods: Cu-64 radioisotope was bound to silica coated SPION using electrolysis plating with tin & palladium seeding. Cellular uptake of Cu-64 SPION was facilitated with a transfecting agent. Functional assays including 51Chromium release, cytometric bead array demonstrated that labelling proce..View full abstract