High-throughput multiplex qPCRs for the surveillance of zoonotic species of canine hookworms
Luca Massetti, Vito Colella, Patsy A Zendejas, Dinh Ng-Nguyen, Lana Harriott, Lara Marwedel, Anke Wiethoelter, Rebecca J Traub
PLOS NEGLECTED TROPICAL DISEASES | PUBLIC LIBRARY SCIENCE | Published : 2020
The canine hookworms Ancylostoma braziliense, Ancylostoma ceylanicum, Ancylostoma caninum and Uncinaria stenocephala are not only capable of producing morbidity and mortality in dogs but are also neglected tropical zoonoses. Each hookworm species differs considerably in its geographical distribution, life cycle, biology, pathogenic impacts on both canine and human hosts, zoonotic potential, and response to treatment with anthelminthics. Here we describe the development and validation of two Taq-Man based multiplex PCR assays capable of detecting and differentiating all four canine hookworm species in faeces of naturally infected dogs. The analytical sensitivity of both assays was assessed us..View full abstract
Funding for this work was provided by Boehringer Ingelheim Animal Health. Luca Massetti and Patsy A. Zendejas are recipients of Melbourne Research Scholarship from the University of Melbourne. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.