Journal article

High-throughput multiplex qPCRs for the surveillance of zoonotic species of canine hookworms

Luca Massetti, Vito Colella, Patsy A Zendejas, Dinh Ng-Nguyen, Lana Harriott, Lara Marwedel, Anke Wiethoelter, Rebecca J Traub

PLoS Neglected Tropical Diseases | PUBLIC LIBRARY SCIENCE | Published : 2020

Abstract

The canine hookworms Ancylostoma braziliense, Ancylostoma ceylanicum, Ancylostoma caninum and Uncinaria stenocephala are not only capable of producing morbidity and mortality in dogs but are also neglected tropical zoonoses. Each hookworm species differs considerably in its geographical distribution, life cycle, biology, pathogenic impacts on both canine and human hosts, zoonotic potential, and response to treatment with anthelminthics. Here we describe the development and validation of two Taq-Man based multiplex PCR assays capable of detecting and differentiating all four canine hookworm species in faeces of naturally infected dogs. The analytical sensitivity of both assays was assessed us..

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