Journal article

Recurrent horizontal transfer identifies mitochondrial positive selection in a transmissible cancer

Andrea Strakova, Thomas J Nicholls, Adrian Baez-Ortega, Maire Ni Leathlobhair, Alexander T Sampson, Katherine Hughes, Isobelle AG Bolton, Kevin Gori, Jinhong Wang, Ilona Airikkala-Otter, Janice L Allen, Karen M Allum, Clara L Arnold, Leontine Bansse-Issa, Thinlay N Bhutia, Jocelyn L Bisson, Kelli Blank, Cristobal Briceno, Artemio Castillo Domracheva, Anne M Corrigan Show all

Nature Communications | NATURE PUBLISHING GROUP | Published : 2020

Abstract

Autonomous replication and segregation of mitochondrial DNA (mtDNA) creates the potential for evolutionary conflict driven by emergence of haplotypes under positive selection for 'selfish' traits, such as replicative advantage. However, few cases of this phenomenon arising within natural populations have been described. Here, we survey the frequency of mtDNA horizontal transfer within the canine transmissible venereal tumour (CTVT), a contagious cancer clone that occasionally acquires mtDNA from its hosts. Remarkably, one canine mtDNA haplotype, A1d1a, has repeatedly and recently colonised CTVT cells, recurrently replacing incumbent CTVT haplotypes. An A1d1a control region polymorphism predi..

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University of Melbourne Researchers

Grants

Awarded by Wellcome


Awarded by EMBO Short-Term Fellowship


Awarded by Sir Henry Dale Fellowship - Wellcome Trust


Awarded by Sir Henry Dale Fellowship - Royal Society


Awarded by Rosetrees and Stoneygate Trust Research Fellowship


Awarded by Medical Research Council Mitochondrial Biology Unit


Funding Acknowledgements

This work was supported by a grant from Wellcome (102942/Z/13/A) and by a Philip Leverhulme Prize from the Leverhulme Trust. A.St. was supported by a Postgraduate Student Award from the Kennel Club Charitable Trust, by an EMBO Short-Term Fellowship (7761) and by the Ruth Bowden Scholarship from the British Federation of Women Graduates. T.J.N. is the recipient of a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (213464/Z/18/Z) and a Rosetrees and Stoneygate Trust Research Fellowship (M811). The work was also supported by grants from Swedish Research Council (M.Fal. and C.M.G.), Swedish Cancer Foundation (M.Fal. and C.M.G.), European Research Council (M.Fal.) and the Knut and Alice Wallenberg Foundation (M.Fal. and C.M.G.). P.F.C. is a Wellcome Trust Principal Research Fellow (212219/Z/18/Z), and a UK NIHR Senior Investigator, who receives support from the Medical Research Council Mitochondrial Biology Unit (MC_UP_1501/2), the Evelyn Trust and the National Institute for Health Research (NIHR) Biomedical Research Centre based at Cambridge University Hospitals, NHS Foundation Trust and the University of Cambridge. The views expressed are those of the authors and not necessarily those of the NHS, the NIHR or the Department of Health. We acknowledge the Core Sequencing Facility, IT groups and members of the Cancer Genome Project at the Wellcome Sanger Institute. We are grateful to Michael R. Stratton for institutional support. We thank Hansong Ma, Michal Minczuk, Robin Weiss, Maximilian R. Stammnitz, Young Mi Kwon and members of the Transmissible Cancer Group for helpful discussions. We appreciate the laboratory support from Bradley Peter, Hector Diaz and other members of the Gustafsson and Falkenberg laboratories. We thank the following individuals for useful information and for their help obtaining samples for this project: Juliana Alzate-Ocampo, Diana Arguello, Jose Ignacio Arias, Sue Barrass, Ekaterina Batrakova, Rafaela Bortolotti Viera, Nikki Brown, Fernando Constantino Casas, John Cooper, Amici Cannis Cotacachi, Johan de Vos, Lytvynenko Dmytro, Phillip Farnham, Ariberto Fassati, Andres Fernandez-Riomalo, Ricardo Gaitan, David Hanzliek, Rafael Ricardo Huppes, John M. Igundu, Matilde Jimenez-Coello, Debra Kamstock, Patrick Kelly, Anna Klucnika, Tatiana Korytina, Anna Kuznetsova, Gleidice Eunice Lavalle (Universidade Federal de Minas Gerais), Olakunle AbdulRasaq Lawal, Thabo Lerotholi, Marco Lima-Maigua, Jimmy Loayza-Feijoo, Mayra Lopez-Bucheli, Mwangi Maina, Margarita Mancero-Albuja, Cynthia Marchiori Bueno, Luis Martinez-Lopez, Alfredo Martinez-Meza, Talita Mariana Morata Raposo, Jude Mulholland, Claudio Murgia, Alvira Murison Swartz, Fran Nargi, Marsden M. Onsare, Edwin Ortiz-Rodriguez, Elisabeth Peach, Lisa Pellegrini, Gerry Polton, Freddy Proano-Perez, Cameron Raw, Ceseltina Semedo, Sanjay Singh, Ivan Stoikov, Mirela Tinucci Costa, Emily Turitto, M. Rifat Vural, David Walker, Kevin Xie, Maurice Zandvliet, staff at Animal Medical Centre Belize City (Belize), veterinary surgeons and staff at Help in Suffering (Jaipur, India), staff at Hopkins Belize Humane Society (Belize), veterinary workers at Pet Centre (UVAS, Lahore, Pakistan), students from St. George's University (True Blue, Grenada, West Indies) who assisted with sample collection, staff at Veterinary Clinic 'El Roble' (Chile), staff and volunteers at World Vets (Gig Harbor, USA) and staff at the WVS International Training Centre in Ooty (India).We are grateful to the following organisations for helpful information: American College of Veterinary Internal Medicine (ACVIM), Animal Balance, Animal Care Association (The Gambia), Animal Management in Rural and Remote Indigenous Communities (AMRRIC), Associacao Bons Amigos de Cabo Verde, Humane Society of Cozumel, Humane Society Veterinary Medical Association-Rural Area Veterinary Services (HSVMA-RAVS), Israel Veterinary Medical Association, Italian Veterinary Oncology Society, Rural Vets South Africa, Veterinary Cancer Society, Veterinary Society of Surgical Oncology (VSSO), VetPharma, Vets Beyond Borders, ViDAS and Coco's Animal Welfare, The Spanky Project, VWB/VSF Canada, West Arnhem Land Dog Health Programme (WALDHeP), World Small Animal Veterinary Association (WSAVA) and (sic) (World Veterinary Medicine). The map in Fig. 1c and Supplementary Fig. 4 was used under the Royalty Free Licence from Free Vector Maps.