Therapeutic Reversal of Radiotherapy Injury to Pro-fibrotic Dysfunctional Fibroblasts In Vitro Using Adipose-derived Stem Cells
Lipi Shukla, Rodney Luwor, Matthew E Ritchie, Shiva Akbarzadeh, Hong-Jian Zhu, Wayne Morrison, Tara Karnezis, Ramin Shayan
PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN | LIPPINCOTT WILLIAMS & WILKINS | Published : 2020
Cancer patients often require radiotherapy (RTx) to enhance their survival. Unfortunately, RTx also damages nearby healthy non-cancer tissues, leading to progressive fibrotic soft-tissue injury, consisting of pain, contracture, tissue-breakdown, infection, and lymphoedema. Mechanisms underlying the clinically observed ability of fat grafting to ameliorate some of these effects, however, are poorly understood. It was hypothesized that RTx significantly alters fibroblast cell function and the paracrine secretome of adipose-derived stem cells (ADSC) may mitigate these changes. Methods: To investigate cellular changes resulting in the fibrotic side-effects of RTx, cultured normal human dermal fi..View full abstract
The O'Brien Institute acknowledges funding support from the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program, Royal Australasian College of Surgeons Foundation for Surgery and Tour de Cure Scholarship, The Wicking Foundation, ACU AORTEC, Stafford Fox Medical Research Foundation, Australasian Foundation for Plastic Surgery, LEW Carty Charitable Fund, Joe White Bequest and the Viertel Charitable Foundation. None of the authors has a financial interest in any of the products, devices, or drugs mentioned in this manuscript.