Journal article

Transcriptional repression of Myc underlies the tumour suppressor function of AGO1 in Drosophila

Olga Zaytseva, Naomi C Mitchell, Linna Guo, Owen J Marshall, Linda M Parsons, Ross D Hannan, David L Levens, Leonie M Quinn

Development (Cambridge) | COMPANY BIOLOGISTS LTD | Published : 2020


Here, we report novel tumour suppressor activity for the Drosophila Argonaute family RNA-binding protein AGO1, a component of the miRNA-dependent RNA-induced silencing complex (RISC). The mechanism for growth inhibition does not, however, involve canonical roles as part of the RISC; rather, AGO1 controls cell and tissue growth by functioning as a direct transcriptional repressor of the master regulator of growth, Myc. AGO1 depletion in wing imaginal discs drives a significant increase in ribosome biogenesis, nucleolar expansion and cell growth in a manner dependent on Myc abundance. Moreover, increased Myc promoter activity and elevated Myc mRNA in AGO1-depleted animals requires RNA polymera..

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Awarded by National Health and Medical Research Council

Funding Acknowledgements

This work was supported by the National Health and Medical Research Council (APP1143008 to L.M.Q.). Deposited in PMC for immediate release.