Journal article
Enhanced Antibacterial Activity of Se Nanoparticles Upon Coating with Recombinant Spider Silk Protein eADF4(kappa 16)
Tao Huang, Sushma Kumari, Heike Herold, Hendrik Bargel, Tamara B Aigner, Daniel E Heath, Neil M O'Brien-Simpson, Andrea J O'Connor, Thomas Scheibel
International Journal of Nanomedicine | Dove Medical Press | Published : 2020
DOI: 10.2147/IJN.S255833
Abstract
Purpose: Selenium nanoparticles (Se NPs) are promising antibacterial agents to tackle the growing problem of antimicrobial resistance. The aim of this study was to fabricate Se NPs with a net positive charge to enhance their antibacterial efficacy. Methods: Se NPs were coated with a positively charged protein--recombinant spider silk protein eADF4([kappa] 16)--to give them a net positive surface charge. Their cytotoxicity and antibacterial activity were investigated, with negatively charged polyvinyl alcohol coated Se NPs as a control. Besides, these eADF4([kappa] 16)-coated Se NPs were immobilized on the spider silk films, and the antibacterial activity of these films was investigated. Resu..
View full abstractGrants
Awarded by European Union Grand ETZ-EFRE 2014-2020, Freistaat Bayern-Tschechien
Awarded by Bavarian Research Foundation
Funding Acknowledgements
The authors acknowledge financial support by the German Academic Exchange service (DAAD) through its Thematic Network Melbourne-Bayreuth Polymer/Colloid Network sponsored from funds of the Federal Ministry of Education and Research (BMBF), and European Union Grand ETZ-EFRE 2014-2020, Freistaat Bayern-Tschechien, Project Nr. 123. Bavarian Research Foundation for financial support (DOK-175-15, T.B.A). TH gratefully acknowledges the support of the University of Melbourne and an Australian Government Research Training Program Scholarship (Melbourne International Research Scholarship).