The Antimicrobial Domains of Wheat Puroindolines Are Cell-Penetrating Peptides with Possible Intracellular Mechanisms of Action
Rebecca L Alfred, Enzo A Palombo, Joseph F Panozzo, Mrinal Bhave
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2013
The puroindoline proteins (PINA and PINB) of wheat display lipid-binding properties which affect the grain texture, a critical parameter for wheat quality. Interestingly, the same proteins also display antibacterial and antifungal properties, attributed mainly to their Tryptophan-rich domain (TRD). Synthetic peptides based on this domain also display selectivity towards bacterial and fungal cells and do not cause haemolysis of mammalian cells. However, the mechanisms of these activities are unclear, thus limiting our understanding of the in vivo roles of PINs and development of novel applications. This study investigated the mechanisms of antimicrobial activities of synthetic peptides based ..View full abstract
Awarded by Australian Research Council (ARC) Linkage Grant
This research was supported by the Australian Research Council (ARC) Linkage Grant LP0989191. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.