Journal article

Measurement of alpha-amylase activity in white wheat flour, milled malt, and microbial enzyme preparations, using the ceralpha assay: Collaborative study

BV McCleary, M McNally, D Monaghan, DC Mugford, C Black, R Broadbent, M Chin, M Cormack, R Fox, C Gaines, P Gothard, S Home, E Howes, C Johnson, R Keeping, M Koliatsou, M Lindhauer, RM de Sa, R Martin, D Monaghan Show all

Journal of AOAC INTERNATIONAL | AOAC INTERNATIONAL | Published : 2002

Abstract

This study was conducted to evaluate the method performance of a rapid procedure for the measurement of alpha-amylase activity in flours and microbial enzyme preparations. Samples were milled (if necessary) to pass a 0.5 mm sieve and then extracted with a buffer/salt solution, and the extracts were clarified and diluted. Aliquots of diluted extract (containing alpha-amylase) were incubated with substrate mixture under defined conditions of pH, temperature, and time. The substrate used was nonreducing end-blocked p-nitrophenyl maltoheptaoside (BPNPG7) in the presence of excess quantities of thermostable alpha-glucosidase. The blocking group in BPNPG7 prevents hydrolysis of this substrate by e..

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