Journal article
Utilising animal models to evaluate oseltamivir efficacy against influenza A and B viruses with reduced in vitro susceptibility
R Farrukee, CMK Tai, DY Oh, DE Anderson, V Gunalan, M Hibberd, GYF Lau, IG Barr, V von Messling, S Maurer-Stroh, AC Hurt
Plos Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2020
Abstract
The neuraminidase (NA) inhibitor (NAI) oseltamivir (OST) is the most widely used influenza antiviral drug. Several NA amino acid substitutions are reported to reduce viral susceptibility to OST in in vitro assays. However, whether there is a correlation between the level of reduction in susceptibility in vitro and the efficacy of OST against these viruses in vivo is not well understood. In this study, a ferret model was utilised to evaluate OST efficacy against circulating influenza A and B viruses with a range of in vitro generated 50% inhibitory concentrations (IC50) values for OST. OST efficacy against an A(H1N1)pdm09 and an A(H1N1) pdm09 virus with the H275Y substitution in neuraminidase..
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Awarded by Department of Health, Australian Government
Funding Acknowledgements
The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health. We further acknowledge the NHMRC-A.STAR grant (APP1055793) for supporting this research work. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.