Journal article

Targeted RNA sequencing enhances gene expression profiling of ultra-low input samples

Fabiola Curion, Adam E Handel, Moustafa Attar, Giuseppe Gallone, Rory Bowden, M Zameel Cader, Michael B Clark

RNA BIOLOGY | TAYLOR & FRANCIS INC | Published : 2020

Abstract

RNA-seq is the standard method for profiling gene expression in many biological systems. Due to the wide dynamic range and complex nature of the transcriptome, RNA-seq provides an incomplete characterization, especially of lowly expressed genes and transcripts. Targeted RNA sequencing (RNA CaptureSeq) focuses sequencing on genes of interest, providing exquisite sensitivity for transcript detection and quantification. However, uses of CaptureSeq have focused on bulk samples and its performance on very small populations of cells is unknown. Here we show CaptureSeq greatly enhances transcriptomic profiling of target genes in ultra-low-input samples and provides equivalent performance to that on..

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University of Melbourne Researchers

Grants

Awarded by Australian National Health and Medical Research Council Early Career Fellowship


Awarded by Wellcome Trust


Awarded by Innovative Medicines Initiative Joint Undertaking


Funding Acknowledgements

This work was supported by an Australian National Health and Medical Research Council Early Career Fellowship [APP1072662 to M.B.C]; a National Institute for Health Research Clinical Lectureship [to A.H.]; the Wellcome Trust via core funding to the Wellcome Centre for Human Genetics [090532/Z/09/Z, 203141/Z/16/Z]; the National Institute for Health Research (NIHR) Oxford Biomedical Research Centre (BRC); the Oxford Biomedical Research Computing (BMRC) facility (a joint development between the Wellcome Centre for Human Genetics and the BigData Institute supported by Health Data Research UK and the NIHR Oxford Biomedical Research Centre); and the Innovative Medicines Initiative Joint Undertaking under grant agreement [no 115439] (StemBANCC), resources of which are composed of financial contribution from the European Union's Seventh Framework Programme [FP7/2007-2013]. The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR or the Department of Health.