Journal article

Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis

Richard G Lee, Junjie Gao, Stefan J Siira, Anne-Marie Shearwood, Judith A Ermer, Vinzenz Hofferek, James C Mathews, Minghao Zheng, Gavin E Reid, Oliver Rackham, Aleksandra Filipovska

JOURNAL OF CELL SCIENCE | COMPANY BIOLOGISTS LTD | Published : 2020

Abstract

The mitochondrial inner membrane contains a unique phospholipid known as cardiolipin (CL), which stabilises the protein complexes embedded in the membrane and supports its overall structure. Recent evidence indicates that the mitochondrial ribosome may associate with the inner membrane to facilitate co-translational insertion of the hydrophobic oxidative phosphorylation (OXPHOS) proteins into the inner membrane. We generated three mutant knockout cell lines for the CL biosynthesis gene Crls1 to investigate the effects of CL loss on mitochondrial protein synthesis. Reduced CL levels caused altered mitochondrial morphology and transcriptome-wide changes that were accompanied by uncoordinated m..

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Grants

Awarded by National Health and Medical Research Council


Awarded by Australian Research Council


Funding Acknowledgements

This project was supported by fellowships and project grants from the National Health and Medical Research Council (APP1058442, APP1045677, APP1041582, APP1023460, APP1005030, APP1043978 to A.F. and O.R.), the Australian Research Council (DP180101656 to A.F. and O.R. and DP190102464 to G.E.R.), the Cancer Council of Western Australia (to O.R. and A.F.). R.G.L. and J.C.M. are supported by UWA Postgraduate Scholarships and J.G. by a Perron Fellowship.