Journal article

Short-term inhibition of autophagy benefits pancreatic beta-cells by augmenting ether lipids and peroxisomal function, and by countering depletion of n-3 polyunsaturated fatty acids after fat-feeding

Kwan Yi Chu, Natalie Mellet, Le May Thai, Peter J Meikle, Trevor J Biden

Molecular Metabolism | ELSEVIER | Published : 2020

Abstract

OBJECTIVE: Investigations of autophagy in β-cells have usually focused on its homeostatic function. More dynamic roles in inhibiting glucose-stimulated insulin secretion (GSIS), potentially involving remodelling of cellular lipids, have been suggested from in vitro studies but not evaluated in vivo. METHODS: We employed temporally-regulated deletion of the essential autophagy gene, Atg7, in β-cells. Mice were fed chow or high-fat diets (HFD), in conjunction with deletion of Atg7 for the last 3 weeks (short-term model) or 9 weeks (long-term model). Standard in vivo metabolic phenotyping was undertaken, and 450 lipid species in islets quantified ex vivo using mass spectroscopy (MS). MIN6 cells..

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University of Melbourne Researchers

Grants

Funding Acknowledgements

This work was supported by Project Grants and Research Fellowships from the National Health and Medical Research Council of Australia to T.J.B. and P.J.M