Effects of a local auxiliary protein on the two-dimensional affinity of a TCR-peptide MHC interaction
Victoria Junghans, Manto Chouliara, Ana Mafalda Santos, Deborah Hatherley, Jan Petersen, Tommy Dam, Lena M Svensson, Jamie Rossjohn, Simon J Davis, Peter Jonsson
Journal of Cell Science | COMPANY BIOLOGISTS LTD | Published : 2020
The affinity of T-cell receptors (TCRs) for major histocompatibility complex molecules (MHCs) presenting cognate antigens likely determines whether T cells initiate immune responses, or not. There exist few measurements of two-dimensional (2D) TCR-MHC interactions, and the effect of auxiliary proteins on binding is unexplored. Here, Jurkat T-cells expressing the MHC molecule HLA-DQ8-glia-α1 and the ligand of an adhesion protein (rat CD2) were allowed to bind supported lipid bilayers (SLBs) presenting fluorescently labelled L3-12 TCR and rat CD2, allowing measurements of binding unconfounded by cell signaling effects or co-receptor binding. The 2D Kd for L3-12 TCR binding to HLA-DQ8-glia-α1, ..View full abstract
Awarded by European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program
Awarded by Wellcome Trust
P.J. has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 757797). S.J.D. is funded by theWellcome Trust (grant number 098274/Z/12/Z). J.R. is supported by an Australian Research Council Laureate Fellowship.