Journal article

Small RNA fingerprinting of Alzheimer's disease frontal cortex extracellular vesicles and their comparison with peripheral extracellular vesicles

Lesley Cheng, Laura J Vella, Kevin J Barnham, Catriona McLean, Colin L Masters, Andrew F Hill

JOURNAL OF EXTRACELLULAR VESICLES | TAYLOR & FRANCIS LTD | Published : 2020

Abstract

Alzheimer's disease is a progressive neurodegenerative disorder, with the strongest disease-associated changes observed at clinical or end-stage disease. Transcriptomic deregulation of miRNA expression can spread via "horizontal" RNA transfer through extracellular vesicles (EVs) to act in conjunction with proteins, leading to changes in mRNA, which can provide early signals to indicate forthcoming neuropathological changes in the brain. Here, we analysed the small RNA content, in particular, miRNA, contained in brain-derived EVs isolated from the frontal cortex of Alzheimer's subjects (n = 8) and neurological control subjects (n = 9). Brain-derived EVs were found to contain an upregulation o..

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Grants

Awarded by National Health and Medical Research Council


Funding Acknowledgements

This work was supported by the National Health and Medical Research Council [GNT1041413 to AFH; GNT1132604 to AFH, CLM and KJB] and the Dementia Collaborative Research Centre Research Grant [AFH and LC]. The collection and processing of the brain tissue which has been used for multiple projects was funded by the Mason Foundation awarded to Andrew Watt and Kevin J. Barnham; Dementia Collaborative Research Centres, Australia.