Collagen-An Important Fish Allergen for Improved Diagnosis
Tanja Kalic, Sandip D Kamath, Thimo Ruethers, Aya C Taki, Roni Nugraha, Thu TK Le, Piotr Humeniuk, Nicholas A Williamson, Diamond Hira, Jennifer M Rolland, Robyn E O'Hehir, Danyi Dai, Dianne E Campbell, Heimo Breiteneder, Andreas L Lopata
The Journal of Allergy and Clinical Immunology In Practice | ELSEVIER | Published : 2020
Background Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen is not fully appreciated. This is likely due to collagen insolubility in neutral aqueous solutions, leading to low abundance in commercially available in vitro and skin prick tests for fish allergy. Objective To investigate the relevance of fish collagen as an allergen in a large patient population (n = 101). Methods Acid-soluble collagen type I was extracted from muscle and skin of Atlantic salmon, barramundi, and yellowfin tuna. IgE binding to collagen was analyzed by ELISA for 101 fish-allergic patients. Collagen-sensitized patients' sera were tested for ..View full abstract
Awarded by National Health and Medical Research Council
Awarded by Austrian Science Fund
Funding for this research was provided by the Centre for Food and Allergy Research, Murdoch Childrens Research Institute; the National Health and Medical Research Council (project grants nos. GNT1086656 and GNT1124143); the Austrian Science Fund (project grant W1248-B30); and the Medical University of Vienna.