Journal article

Combinatorial Single-Cell Analyses of Granulocyte-Monocyte Progenitor Heterogeneity Reveals an Early Uni-potent Neutrophil Progenitor

Immanuel Kwok, Etienne Becht, Yu Xia, Melissa Ng, Ye Chean Teh, Leonard Tan, Maximilien Evrard, Jackson LY Li, Hoa TN Tran, Yingrou Tan, Dehua Liu, Archita Mishra, Ka Hang Liong, Keith Leong, Yuning Zhang, Andre Olsson, Chinmay Kumar Mantri, Pavithra Shyamsunder, Zhaoyuan Liu, Cecile Piot Show all

Immunity | CELL PRESS | Published : 2020

Abstract

Granulocyte-monocyte progenitors (GMPs) have been previously defined for their potential to generate various myeloid progenies such as neutrophils and monocytes. Although studies have proposed lineage heterogeneity within GMPs, it is unclear if committed progenitors already exist among these progenitors and how they may behave differently during inflammation. By combining single-cell transcriptomic and proteomic analyses, we identified the early committed progenitor within the GMPs responsible for the strict production of neutrophils, which we designate as proNeu1. Our dissection of the GMP hierarchy led us to further identify a previously unknown intermediate proNeu2 population. Similar pop..

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Grants

Awarded by Singapore Ministry of Health's National Medical Research Council under its Open Fund -Young Investigator Research Grant


Awarded by Singapore National Research Foundation Investigatorship (NRFI)


Awarded by National Research Foundation (NRF) Singapore


Funding Acknowledgements

We thank all members of L.G.N. laboratory, the SIgN flow cytometry team, the SIgN functional genomics team for their assistance with transcriptomics, and the SIgN mouse core facility for their technical help and support. This research was funded by Singapore Immunology Network (SIgN) core funding and A*STAR, Singapore. S.Z.C. is supported by Singapore Ministry of Health's National Medical Research Council under its Open Fund -Young Investigator Research Grant (OFYIRG17may036). F.G. is an EMBO YIP awardee and is supported by SIgN core funding and by Singapore National Research Foundation Investigatorship (NRFI) (NRF2016NRF-NRFI001-02). L.G.N. is supported by SIgN core funding. SIgN Flow Cytometry facility is supported by National Research Foundation (NRF) Singapore under Shared Infrastructure Support (SIS) (NRF2017_SISFP09).