Book Chapter
Dynamics of MicroRNA Biogenesis
Mohamed Fareh
BIOPHYSICS OF RNA-PROTEIN INTERACTIONS: A MECHANISTIC VIEW | SPRINGER | Published : 2019
Abstract
MicroRNAs (miRNAs) are a class of short non-coding RNAs (~22 nt) that play important gene-regulatory functions in nearly all biological processes in eukaryotic organisms. Mature miRNAs are loaded into an Argonaute (Ago) protein effector and guide the nucleoprotein complex to recognize and silence target RNAs post-transcriptionally. This chapter outlines our current understanding of miRNA biogenesis from its initial transcription to the processing by the microprocessor in the nucleus, to the nuclear export and the cytoplasmic maturation by the endoribonuclease Dicer. This book chapter focuses on recent biophysical and single-molecule studies that have revealed important molecular bases and dy..
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Awarded by Fondation pour la Recherche Medicale
Funding Acknowledgements
I thank Chirlmin Joo and Joe Trapani laboratory members for their help, support, and the insightful discussions. This work was supported by the Fondation pour la Recherche Medicale [SPE20120523964 to Mohamed Fareh]; and the Zero Childhood Cancer initiative in Australia led by Prof. Michelle Haber (executive director, Children's Cancer Institute, Sydney, Australia) and Prof. Joe Trapani (head of Cancer Immunotherapy program, Peter MacCallumCancer Centre, Melbourne, Australia).