Journal article
CELLULOSE SYNTHASE INTERACTING 1 is required for wood mechanics and leaf morphology in aspen
Anne Bunder, Ola Sundman, Amir Mahboubi, Staffan Persson, Shawn D Mansfield, Markus Ruggeberg, Totte Niittyla
PLANT JOURNAL | WILEY | Published : 2020
DOI: 10.1111/tpj.14873
Abstract
Cellulose microfibrils synthesized by CELLULOSE SYNTHASE COMPLEXES (CSCs) are the main load‐bearing polymers in wood. CELLULOSE SYNTHASE INTERACTING1 (CSI1) connects CSCs with cortical microtubules, which align with cellulose microfibrils. Mechanical properties of wood are dependent on cellulose microfibril alignment and structure in the cell walls, but the molecular mechanism(s) defining these features is unknown. Herein, we investigated the role of CSI1 in hybrid aspen (Populus tremula × Populus tremuloides) by characterizing transgenic lines with significantly reduced CSI1 transcript abundance. Reduction in leaves (50–80%) caused leaf twisting and misshaped pavement cells, while reduction..
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Funding Acknowledgements
We thank Cheng Choo Lee and Agnieszka Ziolkowska for assistance with electron microscopy, Junko Takahashi-Schmidt and the biopolymer analytical facility at UPSC for help with wood analysis and Valentina Floran for help with the cloning of the CSI1RNAi construct. This work was supported by Bio4Energy (Swedish Programme for Renewable Energy), the Umea Plant Science Centre, Berzelii Centre for Forest Biotechnology funded by VINNOVA and the Swedish Research Council for Sustainable Development (Formas). Staffan Persson was supported by R@MAP Professor Funds at University of Melbourne and ARC DP and FT grants (DP190101941; FT160100218).