Journal article

UDP-Api/UDP-Xyl synthases affect plant development by controlling the content of UDP-Api to regulate the RG-II-borate complex

Xianhai Zhao, Berit Ebert, Baocai Zhang, Huabin Liu, Yutao Zhang, Wei Zeng, Carsten Rautengarten, Huiling Li, Xiaoyang Chen, Antony Bacic, Guodong Wang, Shuzhen Men, Yihua Zhou, Joshua L Heazlewood, Ai-Min Wu

PLANT JOURNAL | WILEY | Published : 2020

Abstract

Rhamnogalacturonan‐II (RG‐II) is structurally the most complex glycan in higher plants, containing 13 different sugars and 21 distinct glycosidic linkages. Two monomeric RG‐II molecules can form an RG‐II‐borate diester dimer through the two apiosyl (Api) residues of side chain A to regulate cross‐linking of pectin in the cell wall. But the relationship of Api biosynthesis and RG‐II dimer is still unclear. In this study we investigated the two homologous UDP‐D‐apiose/UDP‐D‐xylose synthases (AXSs) in Arabidopsis thaliana that synthesize UDP‐D‐apiose (UDP‐Api). Both AXSs are ubiquitously expressed, while AXS2 has higher overall expression than AXS1 in the tissues analyzed. The homozygous axs do..

View full abstract

Grants

Awarded by National Natural Science Foundation of China


Awarded by Guangdong Province Science and Technology Projects


Awarded by Australian Research Council


Funding Acknowledgements

This work was supported by the National Natural Science Foundation of China (31670670, 31870653, and 31811530009) and the Guangdong Province Science and Technology Projects (2015A050502045 and 2016A010104012) to AMW. The work was supported in part by an Australian Research Council Discovery Project (DP180102630) to JLH. BE is supported by a Future Fellowship from the Australian Research Council (FT160100276). WZ and AB acknowledge the support of an Australian Research Council grant to the ARC Centre of Excellence in Plant Cell Walls (CE110001007).