Journal article

Chemokine receptor CXCR7 non-cell-autonomously controls pontine neuronal migration and nucleus formation

Yan Zhu, Tatsumi Hirata, Fabienne Mackay, Fujio Murakami

Scientific Reports | NATURE PUBLISHING GROUP | Published : 2020

Abstract

Long distance tangential migration transports neurons from their birth places to distant destinations to be incorporated into neuronal circuits. How neuronal migration is guided during these long journeys is still not fully understood. We address this issue by studying the migration of pontine nucleus (PN) neurons in the mouse hindbrain. PN neurons migrate from the lower rhombic lip first anteriorly and then turn ventrally near the trigeminal ganglion root towards the anterior ventral hindbrain. Previously we showed that in mouse depleted of chemokine receptor CXCR4 or its ligand CXCL12, PN neurons make their anterior-to-ventral turn at posteriorized positions. However, the mechanism that sp..

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University of Melbourne Researchers

Grants

Awarded by Ministry of Education, Culture, Science and Technology, Japan


Funding Acknowledgements

We thank Dr. Ryoichiro Kageyama for Nestin-Cre transgenic mice; Dr. Tetsuichiro Saito for BARHL1 in situ construct; Dr. Roger Tsien for mCherry construct, Dr. Yasuto Tanabe for pCAGGS-NLS-EGFP and pCAGGS-NLS-Cre constructs, and Dr. Kenta Yamauchi for pCALNL5-eGFP construct. We also thank Mr. Koki Shirosaki for Cxcr7 in situ hybridization on sections. We are grateful for the technical support from Mr. Xun Hong and Dr. Yi Wang, who were supported by an Osaka University Program for the Support of Networking among Present and Future Researchers, and Ms. Aki Masuda from National Institute of Genetics. We appreciate Dr. Kazuhiko Nishida for critical reading of this manuscript. We thank ChemoCentryx, Mountain View, CA, USA, for the generous gift of small molecule modulators of CXCR7. This work was funded by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Science and Technology, Japan; Contract Grant Nos. 23570226, 16K07010, 20K06865 (to Y.Z.), and 2222004 (to F. Murakami). The authors declare no competing financial interest.