Journal article
Comparative performance of the BGI and Illumina sequencing technology for single-cell RNA-sequencing.
Anne Senabouth, Stacey Andersen, Qianyu Shi, Lei Shi, Feng Jiang, Wenwei Zhang, Kristof Wing, Maciej Daniszewski, Samuel W Lukowski, Sandy SC Hung, Quan Nguyen, Lynn Fink, Anthony Beckhouse, Alice Pébay, Alex W Hewitt, Joseph E Powell
NAR Genom Bioinform | Oxford University Press (OUP) | Published : 2020
Abstract
The libraries generated by high-throughput single cell RNA-sequencing (scRNA-seq) platforms such as the Chromium from 10× Genomics require considerable amounts of sequencing, typically due to the large number of cells. The ability to use these data to address biological questions is directly impacted by the quality of the sequence data. Here we have compared the performance of the Illumina NextSeq 500 and NovaSeq 6000 against the BGI MGISEQ-2000 platform using identical Single Cell 3' libraries consisting of over 70 000 cells generated on the 10× Genomics Chromium platform. Our results demonstrate a highly comparable performance between the NovaSeq 6000 and MGISEQ-2000 in sequencing quality,..
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