Journal article
Comparative sequence and methylation analysis of chloroplast and amyloplast genomes from rice
K Muniandy, MH Tan, BK Song, Q Ayub, S Rahman
Plant Molecular Biology | SPRINGER | Published : 2019
Abstract
Key message: Grain amyloplast and leaf chloroplast DNA sequences are identical in rice plants but are differentially methylated. The leaf chloroplast DNA becomes more methylated as the rice plant ages. Abstract: Rice is an important crop worldwide. Chloroplasts and amyloplasts are critical organelles but the amyloplast genome is poorly studied. We have characterised the sequence and methylation of grain amyloplast DNA and leaf chloroplast DNA in rice. We have also analysed the changes in methylation patterns in the chloroplast DNA as the rice plant ages. Total genomic DNA from grain, old leaf and young leaf tissues were extracted from the Oryza sativa ssp. indica cv. MR219 and sequenced usin..
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Awarded by Ministry of Higher Education, Malaysia
Funding Acknowledgements
This work was supported by the School of Science, Monash University Malaysia postgraduate research grant and in part from grants FRGS/1/2013/ST04/MUSM/01/1 and 02-02010-SF0114 from Ministry of Higher Education (MOHE) and Ministry of Science, Technology and Innovation (MOSTI) of Malaysia respectively. We are grateful to Monash University Malaysia Genomics Facility for the sequencing services and technical assistance. We are also thankful to Prof Wickneswari Ratnam, Universiti Kebangsaan Malaysia (UKM) for providing the Oryza sativa ssp. indica cv. MR219 seeds.