Journal article

Cytosine methylation of rice mitochondrial DNA from grain and leaf tissues

Kanagesswari Muniandy, Mun Hua Tan, Saiyara Shehnaz, Beng Kah Song, Qasim Ayub, Sadequr Rahman

Planta | SPRINGER | Published : 2020

Abstract

MAIN CONCLUSION: The rice leaf mitochondrial DNA is  more methylated compared to the rice grain mitochondrial DNA. The old rice leaf mitochondrial DNA has also a higher methylation level than the young rice leaf mitochondrial DNA. The presence of DNA methylation in rice organelles has not been well characterized. We have previously shown that cytosine methylation of chloroplast DNA is different between leaf and grain, and varies between young and old leaves in rice. However, the variation in cytosine methylation of mitochondrial DNA is still poorly characterized. In this study, we have investigated cytosine methylation of mitochondrial DNA in the rice grain and leaf. Based on CpG, CHG, and C..

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University of Melbourne Researchers

Grants

Awarded by Malaysian Ministry of Higher Education (MOHE)


Awarded by Malaysian Ministry of Science, Technology and Innovation (MOSTI)


Funding Acknowledgements

The project was funded by School of Science, Monash University Malaysia postgraduate research grant and partly by Malaysian Ministry of Higher Education (MOHE) (FRGS/1/2013/ST04/MUSM/01/1) and Malaysian Ministry of Science, Technology and Innovation (MOSTI) (02-02010-SF0114), respectively. We are thankful to Monash University Malaysia Genomics Facility for the sequencing services and technical assistance. We also express our gratitude to Prof Wickneswari Ratnam, Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor, Malaysia for providing the Oryza sativa ssp. indica cv. MR219 seeds.