Revealing the Proteome of Motor Cortex Derived Extracellular Vesicles Isolated from Amyotrophic Lateral Sclerosis Human Postmortem Tissues
Natasha Vassileff, Laura J Vella, Harinda Rajapaksha, Mitch Shambrook, Amirmohammad Nasiri Kenari, Catriona McLean, Andrew F Hill, Lesley Cheng
CELLS | MDPI | Published : 2020
Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease characterized by the deposition of misfolded proteins in the motor cortex and motor neurons. Although a multitude of ALS-associated mutated proteins have been identified, several have been linked to small extracellular vesicles such as exosomes involved in cell-cell communication. This study aims to determine the proteome of extracellular vesicles isolated from the motor cortex of ALS subjects and to identify novel ALS-associated deregulated proteins. Motor cortex extracellular vesicles (MCEVs) were isolated from human postmortem ALS (n = 10) and neurological control (NC, n = 5) motor cortex brain tissues and the MCEVs protei..View full abstract
Motor Neuron Disease Foundation Australia and the National Health and Medical Research Council.