Journal article

Parity reduces mammary repopulating activity but does not affect mammary stem cells defined as CD24 CD29/CD49fhi in mice

Genevieve Dall, Jessica Vieusseux, Yashar Seyed-Razavi, Nathan Godde, Mandy Ludford-Menting, Sarah M Russell, Alan Ashworth, Robin L Anderson, Gail P Risbridger, Mark Shackleton, Kara L Britt



BACKGROUND: Breast cancer (BCa) mortality is decreasing with early detection and improvement in therapies. The incidence of BCa, however, continues to increase, particularly estrogen-receptor-positive (ER +) subtypes. One of the greatest modifiers of ER + BCa risk is childbearing (parity), with BCa risk halved in young multiparous mothers. Despite convincing epidemiological data, the biology that underpins this protection remains unclear. Parity-induced protection has been postulated to be due to a decrease in mammary stem cells (MaSCs); however, reports to date have provided conflicting data. METHODS: We have completed rigorous functional testing of repopulating activity in parous mice usin..

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Funding Acknowledgements

This research was supported by an Australian Postgraduate Award scholarship for GD, Pfizer Australia and Victorian Cancer Agency (VCA) Clinical Research Fellowships for MS, an NBCF Early Career fellowship, NHMRC New Investigator grant and VCA Early Career fellowship for KB, and the Australian Cancer Research Foundation (for the Peter Mac Flow Cytometry and Centre for Advanced Histology and Microscopy core facilities). We thank the following Peter MacCallum Cancer Centre core facilities for provision of instrumentation, training and general support: Flow Cytometry, Centre for Advanced Histology and Microscopy and Animal House core facilities.