Requirement for cleavage factor IIm in the control of alternative polyadenylation in breast cancer cells
Rachael E Turner, Lee M Henneken, Marije Liem-Weits, Paul F Harrison, Angavai Swaminathan, Robert Vary, Iva Nikolic, Kaylene J Simpson, David R Powell, Traude H Beilharz, Bernhard Dichtl
RNA | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | Published : 2020
Alternative polyadenylation (APA) determines stability, localization and translation potential of the majority of mRNA in eukaryotic cells. The heterodimeric mammalian cleavage factor II (CF IIm) is required for pre-mRNA 3' end cleavage and is composed of the RNA kinase hClp1 and the termination factor hPcf11; the latter protein binds to RNA and the RNA polymerase II carboxy-terminal domain. Here, we used siRNA mediated knockdown and poly(A) targeted RNA sequencing to analyze the role of CF IIm in gene expression and APA in estrogen receptor positive MCF7 breast cancer cells. Identified gene ontology terms link CF IIm function to regulation of growth factor activity, protein heterodimerizati..View full abstract
Awarded by NHMRC
Awarded by ARC
We thank members of the Beilharz laboratory for constructive comments on the manuscript, Michael Ming See from the Monash Bioinformatics Platform for technical assistance and Yasmin Saab for help with the sucrose cushion analysis. The Victorian Centre for Functional Genomics (K.J.S.) is funded by the Australian Cancer Research Foundation (ACRF), the Australian Phenomics Network (APN) through funding from the Australian Government's National Collaborative Research Infrastructure Strategy (NCRIS) program and the Peter MacCallum Cancer Centre Foundation. T.H.B. was supported by a Monash Bio Discovery Fellowship and research in the Beilharz Laboratory was funded by grants from the NHMRC (APP1128250) and the ARC (DP170100569 and FT180100049).