Journal article

Leukocyte Tetraspanin CD53 Restrains alpha(3) Integrin Mobilization and Facilitates Cytoskeletal Remodeling and Transmigration in Mice

Louisa Yeung, Jeremy ML Anderson, Janet L Wee, Maria C Demaria, Michaela Finsterbusch, Yuxin S Liu, Pam Hall, Brodie C Smith, Wendy Dankers, Kirstin D Elgass, Ian P Wicks, Hang Fai Kwok, Mark D Wright, Michael J Hickey

JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2020

Abstract

The importance of tetraspanin proteins in regulating migration has been demonstrated in many diverse cellular systems. However, the function of the leukocyte-restricted tetraspanin CD53 remains obscure. We therefore hypothesized that CD53 plays a role in regulating leukocyte recruitment and tested this hypothesis by examining responses of CD53-deficient mice to a range of inflammatory stimuli. Deletion of CD53 significantly reduced neutrophil recruitment to the acutely inflamed peritoneal cavity. Intravital microscopy revealed that in response to several inflammatory and chemotactic stimuli, absence of CD53 had only minor effects on leukocyte rolling and adhesion in postcapillary venules. In..

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Grants

Awarded by National Health and Medical Research Council (NHMRC) Australia


Awarded by NHMRC Medical Research Future Fund Practitioner Fellowship


Awarded by Austrian Science Fund Erwin Schroedinger Fellowship


Awarded by Science and Technology Development Fund, Macau Special Administrative Region


Funding Acknowledgements

This work was supported by funding from National Health and Medical Research Council (NHMRC) Australia Project Grant 1033198 (to M.D.W.), Program Grant 1113577 (to I.P.W.), Senior Research Fellowship 1042775 (to M.J.H.), NHMRC Medical Research Future Fund Practitioner Fellowship 1154325 (to I.P.W.), The Reid Charitable Trusts (to I.P.W.), the Rebecca L. Cooper Medical Research Foundation and ANZ Trustees (to M.J.H.), Austrian Science Fund Erwin Schroedinger Fellowship J 3752-B28, (to M.F.), and funding from the Science and Technology Development Fund, Macau Special Administrative Region (0055/2019/A1; to H.F.K.). This study was made possible through Victorian State Government Operational Infrastructure Support and the Australian Government NHMRC Independent Research Institute Infrastructure Support scheme.