Membrane budding is a major mechanism of in vivo platelet biogenesis

Kathryn S Potts; Alison Farley; Caleb A Dawson; Joel Rimes; Christine Biben; Carolyn de Graaf; Margaret A Potts; Olivia J Stonehouse; Amandine Carmagnac; Pradnya Gangatirkar; Emma C Josefsson; Casey Anttila; Daniela Amann-Zalcenstein; Shalin Naik; Warren S Alexander; Douglas J Hilton; Edwin D Hawkins; Samir Taoudi

Journal article

Membrane budding is a major mechanism of in vivo platelet biogenesis

Kathryn S Potts, Alison Farley, Caleb A Dawson, Joel Rimes, Christine Biben, Carolyn de Graaf, Margaret A Potts, Olivia J Stonehouse, Amandine Carmagnac, Pradnya Gangatirkar, Emma C Josefsson, Casey Anttila, Daniela Amann-Zalcenstein, Shalin Naik, Warren S Alexander, Douglas J Hilton, Edwin D Hawkins, Samir Taoudi

JOURNAL OF EXPERIMENTAL MEDICINE | ROCKEFELLER UNIV PRESS | Published : 2020

DOI: 10.1084/jem.20191206

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Abstract

How platelets are produced by megakaryocytes in vivo remains controversial despite more than a century of investigation. Megakaryocytes readily produce proplatelet structures in vitro; however, visualization of platelet release from proplatelets in vivo has remained elusive. We show that within the native prenatal and adult environments, the frequency and rate of proplatelet formation is incompatible with the physiological demands of platelet replacement. We resolve this inconsistency by performing in-depth analysis of plasma membrane budding, a cellular process that has previously been dismissed as a source of platelet production. Our studies demonstrate that membrane budding results in the..

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University of Melbourne Researchers

Douglas Hilton Author Medical Biology (w.e.h.i.)

Samir Taoudi Author Medical Biology (w.e.h.i.)

Emma Josefsson Author Medical Biology (w.e.h.i.)

Christine Biben Author Medical Biology (w.e.h.i.)

Grants

Awarded by National Health and Medical Research Council

Awarded by National Health andMedical Research Council

Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

This work was supported by the Australian Research Council Stem Cells Australia program, National Health and Medical Research Council project (1128993, 1129012) and Program (1113577) grants, and Independent Research Institutes Infrastructure Support Scheme from the National Health andMedical Research Council (grant 361646), the Australian Cancer Research Foundation, and State Government of Victoria Operational Infrastructure Support. E.D. Hawkins and W.S. Alexander were supported by National Health and Medical Research Council fellowships; K.S. Potts by an Australian Research Council Strategic Research Australian Postgraduate Award studentship; and E.C. Josefsson by a fellowship from the Lorenzo and Pamela Galli Charitable Trust.