Pan-Family Assays for Rapid Viral Screening: Reducing Delays in Public Health Responses During Pandemics.
Michael Erlichster, Gursharan Chana, Daniela Zantomio, Benjamin Goudey, Efstratios Skafidas
Clinical Infectious Diseases | Oxford University Press (OUP) | Published : 2020
BACKGROUND: COVID-19 has highlighted deficiencies in the testing capacity of many developed countries during the early stages of pandemics. Here we describe a strategy utilizing pan-family viral assays to improve early accessibility of large-scale nucleic acid testing. METHODS: Coronaviruses and SARS-CoV-2 were used as a case-study for assessing utility of pan-family viral assays during the early stages of a novel pandemic. Specificity of a pan-coronavirus (Pan-CoV) assay for a novel pathogen was assessed using the frequency of common human coronavirus (HCoV) species in key populations. A reported Pan-CoV assay was assessed to determine sensitivity to 60 reference coronaviruses, including SA..View full abstract