Targeting Antigens to Different Receptors on Conventional Type 1 Dendritic Cells Impacts the Immune Response
Even Fossum, Demo Yemane Tesfaye, Sonja Bobic, Arnar Gudjonsson, Ranveig Braathen, Mireille H Lahoud, Irina Caminschi, Bjarne Bogen
The Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 2020
Targeting Ag to surface receptors on conventional type 1 dendritic cells can enhance induction of Ab and T cell responses. However, it is unclear to what extent the targeted receptor influences the resulting responses. In this study, we target Ag to Xcr1, Clec9A, or DEC-205, surface receptors that are expressed on conventional type 1 dendritic cells, and compare immune responses in BALB/c and C57BL/6 mice in vitro and in vivo after intradermal DNA vaccination. Targeting hemagglutinin from influenza A to Clec9A induced Ab responses with higher avidity that more efficiently neutralized influenza virus compared with Xcr1 and DEC-205 targeting. In contrast, targeting Xcr1 resulted in higher IFN-..View full abstract
Awarded by EURONANOMED European Union
Awarded by Research Council of Norway
This work was supported by the Kristian Gerhard Jebsen Foundation (to B.B.), EURONANOMED European Union Grant 2012023 (to B.B.), and Research Council of Norway Grant 250884 (to E.F.).