Journal article

Targeting Antigens to Different Receptors on Conventional Type 1 Dendritic Cells Impacts the Immune Response

Even Fossum, Demo Yemane Tesfaye, Sonja Bobic, Arnar Gudjonsson, Ranveig Braathen, Mireille H Lahoud, Irina Caminschi, Bjarne Bogen

The Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 2020

Abstract

Targeting Ag to surface receptors on conventional type 1 dendritic cells can enhance induction of Ab and T cell responses. However, it is unclear to what extent the targeted receptor influences the resulting responses. In this study, we target Ag to Xcr1, Clec9A, or DEC-205, surface receptors that are expressed on conventional type 1 dendritic cells, and compare immune responses in BALB/c and C57BL/6 mice in vitro and in vivo after intradermal DNA vaccination. Targeting hemagglutinin from influenza A to Clec9A induced Ab responses with higher avidity that more efficiently neutralized influenza virus compared with Xcr1 and DEC-205 targeting. In contrast, targeting Xcr1 resulted in higher IFN-..

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University of Melbourne Researchers