Journal article

Bcl2-Associated X (BAX) Knockout in Mice Resulted in Persistence of Neonatal Testicular Germ Cells (Gonocytes)

Ruil Li, Shengde Wu, Manpreet Kaur, Shan Zong, Sanduni A Abeydeera, Tuya Bao, Shaista Arif, John M Hutson

Sexual Development | KARGER | Published : 2020

Abstract

During early testicular development, neonatal gonocytes transform into spermatogonial stem cells (SSC), and any untransformed gonocytes are thought to undergo apoptosis. In human cryptorchidism, persisting gonocytes may lead to seminoma. Using Bcl2-associated X knockout (BAXKO) mice, we investigated apoptosis in gonocyte development during mouse minipuberty. Testes from BAXKO, heterozygous (HET), and wild-type (WT) littermates were collected on postnatal days 1, 3, 6, and 9 (n = 6/group), labelled with antibodies against mouse vasa homologue (MVH, germ cell marker) or promyelocytic leukaemia zinc-factor (PLZF, SSC marker) and imaged for cell counting. Total germ cells/tubule, i.e., the numbe..

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Grants

Awarded by Australian National Health and Medical Research Council


Funding Acknowledgements

This study was supported by the Australian National Health and Medical Research Council (Grant Number APP1127109) and the Victorian Government's Operational Infrastructure Support Programme.