Distribution of Particles in Human Stem Cell-Derived 3D Neuronal Cell Models: Effect of Particle Size, Charge, and Density
Ewa Czuba-Wojnilowicz, Sara Miellet, Agata Glab, Serena Viventi, Francesca Cavalieri, Christina Cortez-Jugo, Mirella Dottori, Frank Caruso
BIOMACROMOLECULES | AMER CHEMICAL SOC | Published : 2020
Neurodegenerative diseases are generally characterized by a progressive loss of neuronal subpopulations, with no available cure to date. One of the main reasons for the limited clinical outcomes of new drug formulations is the lack of appropriate in vitro human cell models for research and validation. Stem cell technologies provide an opportunity to address this challenge by using patient-derived cells as a platform to test various drug formulations, including particle-based drug carriers. The therapeutic efficacy of drug delivery systems relies on efficient cellular uptake of the carrier and can be dependent on its size, shape, and surface chemistry. Although considerable efforts have been ..View full abstract
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Awarded by Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology
Awarded by National Health and Medical Research Council Senior Principal Research Fellowship
This research was conducted and funded by the Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology (project number CE140100036). F.C. acknowledges the award of a National Health and Medical Research Council Senior Principal Research Fellowship (GNT1135806). S.V. was funded by a Melbourne International Research Scholarship and a Melbourne International Fee Remission Scholarship (The University of Melbourne). This work was performed in part at the Materials Characterisation and Fabrication Platform (MCFP) at The University of Melbourne and the Victorian Node of the Australian National Fabrication Facility (ANFF) and Melbourne Histology Platform. This study was also supported by funding from the National Ataxia Foundation, Friedreich's Ataxia Research Alliance (USA), Friedreich's Ataxia Research Association Australasia, The University of Melbourne, and University of Wollongong. The experiments with hESC were performed in accordance with the Guidelines of the National Health and Medical Research Council and approved by The University of Melbourne and University of Wollongong Human Ethics Committees (#1545384 and #2017/375).