Kelussia odoratissima Mozaff. activates intrinsic pathway of apoptosis in breast cancer cells associated with S phase cell cycle arrest via involvement of p21/p27 in vitro and in vivo
Hamed Karimian, Aditya Arya, Mehran Fadaeinasab, Mahboubeh Razavi, Maryam Hajrezaei, Ataul Karim Khan, Hapipah Mohd Ali, Mahmood Ameen Abdulla, Mohamad Ibrahim Noordin
Drug Design, Development and Therapy | DOVE MEDICAL PRESS LTD | Published : 2017
The aim of this study was to evaluate the anticancer potential of Kelussia odoratissima. Several in vitro and in vivo biological assays were applied to explore the direct effect of an extract and bioactive compound of this plant against breast cancer cells and its possible mechanism of action.K. odoratissima methanol extract (KME) was prepared, and MTT assay was used to evaluate the cytotoxicity. To identify the cytotoxic compound, a bioassay-guided investigation was performed on methanol extract. 8-Hydroxy-ar-turmerone was isolated as a bioactive compound. In vivo study was performed in the breast cancer rat model. LA7 cell line was used to induce the breast tumor. Histopathological and exp..View full abstract
Awarded by ministry of higher education
All the data generated or analyzed during this study are included in this article. This research work is supported by the fundamental research grant (FRGS): FP021-2014A from the ministry of higher education, and RP043B-15HTM Malaysia.