Induction of Apoptosis in Human Breast Cancer Cells via Caspase Pathway by Vernodalin Isolated from Centratherum anthelminticum (L.) Seeds
Chung Yeng Looi, Aditya Arya, Foo Kit Cheah, Bushra Muharram, Kok Hoong Leong, Khalit Mohamad, Won Fen Wong, Nitika Rai, Mohd Rais Mustafa
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2013
BACKGROUND: Centratherum anthelminticum (L.) seeds (CA) is a well known medicinal herb in Indian sub-continent. We recently reported anti-oxidant property of chloroform fraction of Centratherum anthelminticum (L.) seeds (CACF) by inhibiting tumor necrosis factor-α (TNF-α)-induced growth of human breast cancer cells. However, the active compounds in CACF have not been investigated previously. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we showed that CACF inhibited growth of MCF-7 human breast cancer cells. CACF induced apoptosis in MCF-7 cells as marked by cell size shrinkage, deformed cytoskeletal structure and DNA fragmentation. To identify the cytotoxic compound, CACF was subjected to ..View full abstract
Awarded by University Malaya
Awarded by University Malaya High Impact research grant from Ministry of Higher Education (MOHE), Malaysia
This study was supported by University Malaya research grant (RP001-2012B) and University Malaya High Impact research grant (H-20001-E00002) from Ministry of Higher Education (MOHE), Malaysia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.