Journal article

Spatial and morphological reorganization of endosymbiosis during metamorphosis accommodates adult metabolic requirements in a weevil

Justin Maire, Nicolas Parisot, Mariana Galvao Ferrarini, Agnes Vallier, Benjamin Gillet, Sandrine Hughes, Severine Balmand, Carole Vincent-Monegat, Anna Zaidman-Remy, Abdelaziz Heddi

Proceedings of the National Academy of Sciences | NATL ACAD SCIENCES | Published : 2020

Abstract

Bacterial intracellular symbiosis (endosymbiosis) is widespread in nature and impacts many biological processes. In holometabolous symbiotic insects, metamorphosis entails a complete and abrupt internal reorganization that creates a constraint for endosymbiont transmission from larvae to adults. To assess how endosymbiosis copes-and potentially evolves-throughout this major host-tissue reorganization, we used the association between the cereal weevil Sitophilus oryzae and the bacterium Sodalis pierantonius as a model system. S. pierantonius are contained inside specialized host cells, the bacteriocytes, that group into an organ, the bacteriome. Cereal weevils require metabolic inputs from th..

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Funding Acknowledgements

The authors thank Catherine Fiol, Sergio Lopez-Madrigal, Rita Rebollo, and Carlos Vargas-Chavez for their valuable help on animal rearing and sampling; Fabien Filaire and Sandra Hamamjian for their help in microtomy; Ina Atree and Eric Faudry for kindly reviewing our data on the type III secretion system and discussing with us the conservation of these bacterial genes in Sodalis; and the anonymous reviewers and editor for their constructive comments that helped improve the manuscript. Confocal and electron microscopy was performed at the Centre Technologique des Microstructures. Funding for this project was provided by Institut National de la Recherche Agronomique, Institut National des Sciences AppliqueesLyon, the French ANR-13-BSV7-0016-01 (IMetSym), the French ANR-17_CE20_0031_01 (GREEN), the "Sante des Plantes et Environnement" Institut National de la Recherche Agronomique department, and a grant CMIRA from Region Rhone-Alpes.