Journal article

Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNA

Jean YH Lee, Nickala Best, Julie McAuley, Jessica L Porter, Torsten Seemann, Mark B Schultz, Michelle Sait, Nicole Orlando, Karolina Mercoulia, Susan A Ballard, Julian Druce, Thomas Tran, Mike G Catton, Melinda J Pryor, Huanhuan L Cui, Angela Luttick, Sean McDonald, Arran Greenhalgh, Jason C Kwong, Norelle L Sherry Show all

JOURNAL OF MEDICAL MICROBIOLOGY | MICROBIOLOGY SOC | Published : 2020

Abstract

Introduction. The SARS-CoV-2 pandemic of 2020 has resulted in unparalleled requirements for RNA extraction kits and enzymes required for virus detection, leading to global shortages. This has necessitated the exploration of alternative diagnostic options to alleviate supply chain issues.Aim. To establish and validate a reverse transcription loop-mediated isothermal amplification (RT- LAMP) assay for the detection of SARS-CoV-2 from nasopharyngeal swabs.Methodology. We used a commercial RT-LAMP mastermix from OptiGene in combination with a primer set designed to detect the CDC N1 region of the SARS-CoV-2 nucleocapsid (N) gene. A single-tube, single-step fluorescence assay was implemented wher..

View full abstract

Grants

Awarded by National Health and Medical Research Council (NHMRC) of Australia


Awarded by NHMRC Research Fellowship


Awarded by NHMRC Practitioner Fellowship


Funding Acknowledgements

D.A.W. is supported by an Emerging Leadership Investigator Grant from the National Health and Medical Research Council (NHMRC) of Australia (APP1174555). T.P.S. is supported by NHMRC Research Fellowship (APP1105525). B.P.H. is supported by NHMRC Practitioner Fellowship (APP1105905).