Journal article

Increased antibiotic susceptibility in Neisseria gonorrhoeae through adaptation to the cervical environment

Kevin Ma, Tatum Mortimer, Allison Hicks, Nicole Wheeler, Leonor Sánchez-Busó, Daniel Golparian, George Taiaroa, Daniel HF Rubin, Yi Wang, Deborah Williamson, Magnus Unemo, Simon Harris, Yonatan Grad

Published : 2020

Abstract

Neisseria gonorrhoeae is an urgent public health threat due to rapidly increasing incidence and antibiotic resistance. In contrast with the trend of increasing resistance, clinical isolates that have reverted to susceptibility regularly appear, prompting questions about which pressures compete with antibiotics to shape gonococcal evolution. Here, we used genome-wide association on the largest collection of N. gonorrhoeae isolates to date (n=4852) to identify loss-of-function (LOF) mutations in the efflux pump mtrCDE operon as a mechanism of increased antibiotic susceptibility and demonstrate that these mutations are overrepresented in cervical isolates relative to urethral isolates (odds rat..

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