Dyslipidemic Diabetic Serum Increases Lipid Accumulation and Expression of Stearoyl-CoA Desaturase in Human Macrophages
Bruce XW Wong, Reece A Kyle, Paul C Myhill, Kevin D Croft, Carmel M Quinn, Wendy Jessup, Bu B Yeap
Lipids | WILEY | Published : 2011
Type 2 diabetes and dyslipidemia are risk factors for cardiovascular disease. However, mechanisms by which hypertriglyceridemia influences atherogenesis remain unclear. We examined effects of dyslipidemic diabetic serum on macrophage lipid accumulation as a model of foam cell formation. Normal human macrophages were cultured in media supplemented with 10% serum from non-diabetic normolipidemic or non-diabetic hypercholesterolemic adults versus adults with Type 2 diabetes; diabetes and hypertriglyceridemia; or diabetes and hypercholesterolemia. Exposure to diabetic sera resulted in increased macrophage fatty acids (2-3 fold higher, both saturated and unsaturated). Macrophage expression of CD3..View full abstract
Awarded by National Heart Foundation of Australia
We thank staff from Biochemistry, Specimen Reception and Transfusion Medicine, Fremantle Hospital for their assistance with this project, and the Australian Red Cross Blood Service for providing buffy coats. This work was supported by research Grants from the National Heart Foundation of Australia (G07 P3159), the Raine Medical Foundation of Western Australia, the University of Western Australia and the Fremantle Hospital Medical Research Foundation, Western Australia.