Journal article

Modulation of Macrophage Fatty Acid Content and Composition by Exposure to Dyslipidemic Serum in Vitro

Bruce XW Wong, Reece A Kyle, Kevin D Croft, Carmel M Quinn, Wendy Jessup, Bu B Yeap

Lipids | WILEY | Published : 2011

Abstract

Macrophages in arterial walls accumulate lipids leading to the development of atherosclerotic plaques. However, mechanisms underlying macrophage lipid accumulation and foam cell formation are often studied without accounting for risk factors such as dyslipidemia. We investigated the effect of varying concentrations of triglyceride (TG) within physiological range on macrophage fatty acid (FA) accumulation and expression of cholesterol efflux proteins. Human monocytes were cultured in media supplemented with 10% sera containing low (0.7 mmol/L) to high (1.4 mmol/L) TG. The resulting macrophages were harvested after 10 days for analysis of FA content and composition and expression of genes invo..

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University of Melbourne Researchers

Grants

Awarded by National Heart Foundation of Australia


Funding Acknowledgements

We thank Dr. Paul Chubb, Mary Anne Townsend and the staff of the Biochemistry Department and Specimen Reception Area, Fremantle Hospital and the staff of transfusion medicine, Fremantle Hospital for their assistance with this project. We thank the Australian Red Cross Blood Service for providing buffy coats for our experiments. This work was supported by research grants from the National Heart Foundation of Australia (G 07 P 3159), the Raine Medical Foundation of Western Australia, the University of Western Australia and the Fremantle Hospital Medical Research Foundation.